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polyclonal mouse anti pig igg2 antibody  (Bio-Rad)


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    Bio-Rad polyclonal mouse anti pig igg2 antibody
    Fig. 4. Mapping of pA104R B-cell epitopes. Pooled porcine sera (n = 82), at optimized dilutions of 1:250 (IgM) and 1:500 <t>(IgG),</t> were subjected to peptide-based ELISA. (A) Three IgM immunodominant regions were identified producing a clear increase in antibody titers on day 7 p.i. that remains high at least until day 16 p.i. (B) Two IgG immunodominant regions were likewise identified that produce a clear increase in antibody titers on day 13 p.i. that remains high at least until day 35 p. i. Data are presented as mean ± SD of 3 replicates. The cut-off value (CO) was calculated as mean OD of the negative controls (n = 11). If the mean OD of negative controls was lower than 0.105, the value was considered to be 0.105. Values above the solid black lines (S/CO > 1 IgM and S/CO > 2 IgG) were scored as weakly positive and values above the dotted lines (S/CO > 2 IgM and S/CO > 4 IgG) were scored as strongly positive reactions. Grey bars represent weakly positive porcine anti-pA104R epitopes and black bars represent strongly positive porcine anti-PA104R epitopes. DPI – days post-infection.
    Polyclonal Mouse Anti Pig Igg2 Antibody, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal mouse anti pig igg2 antibody/product/Bio-Rad
    Average 93 stars, based on 53 article reviews
    polyclonal mouse anti pig igg2 antibody - by Bioz Stars, 2026-03
    93/100 stars

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    1) Product Images from "Targeted mutagenesis of the β-strand DNA binding region of African swine fever virus histone-like protein (pA104R) impairs DNA-binding activity and antibody recognition."

    Article Title: Targeted mutagenesis of the β-strand DNA binding region of African swine fever virus histone-like protein (pA104R) impairs DNA-binding activity and antibody recognition.

    Journal: Antiviral research

    doi: 10.1016/j.antiviral.2023.105784

    Fig. 4. Mapping of pA104R B-cell epitopes. Pooled porcine sera (n = 82), at optimized dilutions of 1:250 (IgM) and 1:500 (IgG), were subjected to peptide-based ELISA. (A) Three IgM immunodominant regions were identified producing a clear increase in antibody titers on day 7 p.i. that remains high at least until day 16 p.i. (B) Two IgG immunodominant regions were likewise identified that produce a clear increase in antibody titers on day 13 p.i. that remains high at least until day 35 p. i. Data are presented as mean ± SD of 3 replicates. The cut-off value (CO) was calculated as mean OD of the negative controls (n = 11). If the mean OD of negative controls was lower than 0.105, the value was considered to be 0.105. Values above the solid black lines (S/CO > 1 IgM and S/CO > 2 IgG) were scored as weakly positive and values above the dotted lines (S/CO > 2 IgM and S/CO > 4 IgG) were scored as strongly positive reactions. Grey bars represent weakly positive porcine anti-pA104R epitopes and black bars represent strongly positive porcine anti-PA104R epitopes. DPI – days post-infection.
    Figure Legend Snippet: Fig. 4. Mapping of pA104R B-cell epitopes. Pooled porcine sera (n = 82), at optimized dilutions of 1:250 (IgM) and 1:500 (IgG), were subjected to peptide-based ELISA. (A) Three IgM immunodominant regions were identified producing a clear increase in antibody titers on day 7 p.i. that remains high at least until day 16 p.i. (B) Two IgG immunodominant regions were likewise identified that produce a clear increase in antibody titers on day 13 p.i. that remains high at least until day 35 p. i. Data are presented as mean ± SD of 3 replicates. The cut-off value (CO) was calculated as mean OD of the negative controls (n = 11). If the mean OD of negative controls was lower than 0.105, the value was considered to be 0.105. Values above the solid black lines (S/CO > 1 IgM and S/CO > 2 IgG) were scored as weakly positive and values above the dotted lines (S/CO > 2 IgM and S/CO > 4 IgG) were scored as strongly positive reactions. Grey bars represent weakly positive porcine anti-pA104R epitopes and black bars represent strongly positive porcine anti-PA104R epitopes. DPI – days post-infection.

    Techniques Used: Peptide ELISA, Infection

    Fig. 5. Analysis of anti-pA104R antibodies recognizing linear B-cell epitopes. (A) The IgM antibody determinants identified from infected porcine sera at 7, 10, and 13–16 days post-infection. (B) The IgG antibody determinants identified from infected porcine sera at 13–16, 17–20, and 21–30 days post-infection. Regions of amino acid sequences corresponding to the identified B-cell epitopes are indicated in the schematic diagrams of the pA104R sequence. The percentage of antibody recognition contributed by each individual pA104R epitope is indicated in the pie charts and was calculated according to the following equation: % antibody recognition = 100 × (OD values from individual peptide/sum of the OD values from all peptides). In this calculation, the avidity and affinity of the peptides to the sera were assumed to be similar.
    Figure Legend Snippet: Fig. 5. Analysis of anti-pA104R antibodies recognizing linear B-cell epitopes. (A) The IgM antibody determinants identified from infected porcine sera at 7, 10, and 13–16 days post-infection. (B) The IgG antibody determinants identified from infected porcine sera at 13–16, 17–20, and 21–30 days post-infection. Regions of amino acid sequences corresponding to the identified B-cell epitopes are indicated in the schematic diagrams of the pA104R sequence. The percentage of antibody recognition contributed by each individual pA104R epitope is indicated in the pie charts and was calculated according to the following equation: % antibody recognition = 100 × (OD values from individual peptide/sum of the OD values from all peptides). In this calculation, the avidity and affinity of the peptides to the sera were assumed to be similar.

    Techniques Used: Infection, Sequencing

    Fig. 7. pA104R-specific IgG reactivity (A) pA104R-specific IgG antibody detection. (B) PEP15-specific IgG antibody detection. (C) PEP15-specific IgG1 and IgG2 antibody isotype detection. (D) IgG1/IgG2 ratio calculated for each IgG sample as the OD value of IgG1 divided by the OD value of IgG2. Red solid lines represent medians. DPI = days post-infection.
    Figure Legend Snippet: Fig. 7. pA104R-specific IgG reactivity (A) pA104R-specific IgG antibody detection. (B) PEP15-specific IgG antibody detection. (C) PEP15-specific IgG1 and IgG2 antibody isotype detection. (D) IgG1/IgG2 ratio calculated for each IgG sample as the OD value of IgG1 divided by the OD value of IgG2. Red solid lines represent medians. DPI = days post-infection.

    Techniques Used: Infection

    Fig. 6. PEP-specific IgM and IgG antibody determinants. (A) PEP-specific IgM antibody detection in sera (n = 45) at a dilution of 1:250 was determined by peptide- based ELISA. Sera were categorized into non-infected or days post-infection (DPI) = 0 (n = 10), DPI = 7 (n = 10), DPI = 10 (n = 9), and DPI = 13–16 (n = 16). Red solid lines represent medians. (B) PEP-specific IgG antibody detection in sera (n = 48) at a dilution of 1:500 was determined by peptide-based ELISA. Sera were categorized into non-infected or DPI = 0 (n = 10), DPI = 13–16 (n = 16), DPI = 17–20 (n = 11), and DPI = 21–30 (n = 11). Red solid lines represent medians. (C) The pA104R dimeric structure (PDB 6LMH), generated using UCSF Chimera software. The IgM and IgG antibody determinants are indicated in brown (PEP23), and blue (PEP15).
    Figure Legend Snippet: Fig. 6. PEP-specific IgM and IgG antibody determinants. (A) PEP-specific IgM antibody detection in sera (n = 45) at a dilution of 1:250 was determined by peptide- based ELISA. Sera were categorized into non-infected or days post-infection (DPI) = 0 (n = 10), DPI = 7 (n = 10), DPI = 10 (n = 9), and DPI = 13–16 (n = 16). Red solid lines represent medians. (B) PEP-specific IgG antibody detection in sera (n = 48) at a dilution of 1:500 was determined by peptide-based ELISA. Sera were categorized into non-infected or DPI = 0 (n = 10), DPI = 13–16 (n = 16), DPI = 17–20 (n = 11), and DPI = 21–30 (n = 11). Red solid lines represent medians. (C) The pA104R dimeric structure (PDB 6LMH), generated using UCSF Chimera software. The IgM and IgG antibody determinants are indicated in brown (PEP23), and blue (PEP15).

    Techniques Used: Peptide ELISA, Infection, Generated, Software



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    polyclonal mouse anti pig igg2 antibody  (Bio-Rad)
    93
    Bio-Rad polyclonal mouse anti pig igg2 antibody
    Fig. 4. Mapping of pA104R B-cell epitopes. Pooled porcine sera (n = 82), at optimized dilutions of 1:250 (IgM) and 1:500 <t>(IgG),</t> were subjected to peptide-based ELISA. (A) Three IgM immunodominant regions were identified producing a clear increase in antibody titers on day 7 p.i. that remains high at least until day 16 p.i. (B) Two IgG immunodominant regions were likewise identified that produce a clear increase in antibody titers on day 13 p.i. that remains high at least until day 35 p. i. Data are presented as mean ± SD of 3 replicates. The cut-off value (CO) was calculated as mean OD of the negative controls (n = 11). If the mean OD of negative controls was lower than 0.105, the value was considered to be 0.105. Values above the solid black lines (S/CO > 1 IgM and S/CO > 2 IgG) were scored as weakly positive and values above the dotted lines (S/CO > 2 IgM and S/CO > 4 IgG) were scored as strongly positive reactions. Grey bars represent weakly positive porcine anti-pA104R epitopes and black bars represent strongly positive porcine anti-PA104R epitopes. DPI – days post-infection.
    Polyclonal Mouse Anti Pig Igg2 Antibody, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal mouse anti pig igg2 antibody/product/Bio-Rad
    Average 93 stars, based on 1 article reviews
    polyclonal mouse anti pig igg2 antibody - by Bioz Stars, 2026-03
    93/100 stars
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    Fig. 4. Mapping of pA104R B-cell epitopes. Pooled porcine sera (n = 82), at optimized dilutions of 1:250 (IgM) and 1:500 (IgG), were subjected to peptide-based ELISA. (A) Three IgM immunodominant regions were identified producing a clear increase in antibody titers on day 7 p.i. that remains high at least until day 16 p.i. (B) Two IgG immunodominant regions were likewise identified that produce a clear increase in antibody titers on day 13 p.i. that remains high at least until day 35 p. i. Data are presented as mean ± SD of 3 replicates. The cut-off value (CO) was calculated as mean OD of the negative controls (n = 11). If the mean OD of negative controls was lower than 0.105, the value was considered to be 0.105. Values above the solid black lines (S/CO > 1 IgM and S/CO > 2 IgG) were scored as weakly positive and values above the dotted lines (S/CO > 2 IgM and S/CO > 4 IgG) were scored as strongly positive reactions. Grey bars represent weakly positive porcine anti-pA104R epitopes and black bars represent strongly positive porcine anti-PA104R epitopes. DPI – days post-infection.

    Journal: Antiviral research

    Article Title: Targeted mutagenesis of the β-strand DNA binding region of African swine fever virus histone-like protein (pA104R) impairs DNA-binding activity and antibody recognition.

    doi: 10.1016/j.antiviral.2023.105784

    Figure Lengend Snippet: Fig. 4. Mapping of pA104R B-cell epitopes. Pooled porcine sera (n = 82), at optimized dilutions of 1:250 (IgM) and 1:500 (IgG), were subjected to peptide-based ELISA. (A) Three IgM immunodominant regions were identified producing a clear increase in antibody titers on day 7 p.i. that remains high at least until day 16 p.i. (B) Two IgG immunodominant regions were likewise identified that produce a clear increase in antibody titers on day 13 p.i. that remains high at least until day 35 p. i. Data are presented as mean ± SD of 3 replicates. The cut-off value (CO) was calculated as mean OD of the negative controls (n = 11). If the mean OD of negative controls was lower than 0.105, the value was considered to be 0.105. Values above the solid black lines (S/CO > 1 IgM and S/CO > 2 IgG) were scored as weakly positive and values above the dotted lines (S/CO > 2 IgM and S/CO > 4 IgG) were scored as strongly positive reactions. Grey bars represent weakly positive porcine anti-pA104R epitopes and black bars represent strongly positive porcine anti-PA104R epitopes. DPI – days post-infection.

    Article Snippet: The plates were incubated with 1:200 diluted pig sera in 5% BSA in PBS for 1 h, followed by a blocking step with 5% BSA diluted in PBS for 1 h. The plates were then incubated with the 1:100 diluted polyclonal mouse anti-pig IgG1 antibody clone K139 3C8 (BioRad) or polyclonal mouse anti-pig IgG2 antibody clone K68 Ig2 (1:1000) (Bio-Rad) primary antibodies for 1 h, followed by incubation with the HRP-conjugated goat anti-mouse secondary antibody (1:5000) (BioRad) for an additional hour.

    Techniques: Peptide ELISA, Infection

    Fig. 5. Analysis of anti-pA104R antibodies recognizing linear B-cell epitopes. (A) The IgM antibody determinants identified from infected porcine sera at 7, 10, and 13–16 days post-infection. (B) The IgG antibody determinants identified from infected porcine sera at 13–16, 17–20, and 21–30 days post-infection. Regions of amino acid sequences corresponding to the identified B-cell epitopes are indicated in the schematic diagrams of the pA104R sequence. The percentage of antibody recognition contributed by each individual pA104R epitope is indicated in the pie charts and was calculated according to the following equation: % antibody recognition = 100 × (OD values from individual peptide/sum of the OD values from all peptides). In this calculation, the avidity and affinity of the peptides to the sera were assumed to be similar.

    Journal: Antiviral research

    Article Title: Targeted mutagenesis of the β-strand DNA binding region of African swine fever virus histone-like protein (pA104R) impairs DNA-binding activity and antibody recognition.

    doi: 10.1016/j.antiviral.2023.105784

    Figure Lengend Snippet: Fig. 5. Analysis of anti-pA104R antibodies recognizing linear B-cell epitopes. (A) The IgM antibody determinants identified from infected porcine sera at 7, 10, and 13–16 days post-infection. (B) The IgG antibody determinants identified from infected porcine sera at 13–16, 17–20, and 21–30 days post-infection. Regions of amino acid sequences corresponding to the identified B-cell epitopes are indicated in the schematic diagrams of the pA104R sequence. The percentage of antibody recognition contributed by each individual pA104R epitope is indicated in the pie charts and was calculated according to the following equation: % antibody recognition = 100 × (OD values from individual peptide/sum of the OD values from all peptides). In this calculation, the avidity and affinity of the peptides to the sera were assumed to be similar.

    Article Snippet: The plates were incubated with 1:200 diluted pig sera in 5% BSA in PBS for 1 h, followed by a blocking step with 5% BSA diluted in PBS for 1 h. The plates were then incubated with the 1:100 diluted polyclonal mouse anti-pig IgG1 antibody clone K139 3C8 (BioRad) or polyclonal mouse anti-pig IgG2 antibody clone K68 Ig2 (1:1000) (Bio-Rad) primary antibodies for 1 h, followed by incubation with the HRP-conjugated goat anti-mouse secondary antibody (1:5000) (BioRad) for an additional hour.

    Techniques: Infection, Sequencing

    Fig. 7. pA104R-specific IgG reactivity (A) pA104R-specific IgG antibody detection. (B) PEP15-specific IgG antibody detection. (C) PEP15-specific IgG1 and IgG2 antibody isotype detection. (D) IgG1/IgG2 ratio calculated for each IgG sample as the OD value of IgG1 divided by the OD value of IgG2. Red solid lines represent medians. DPI = days post-infection.

    Journal: Antiviral research

    Article Title: Targeted mutagenesis of the β-strand DNA binding region of African swine fever virus histone-like protein (pA104R) impairs DNA-binding activity and antibody recognition.

    doi: 10.1016/j.antiviral.2023.105784

    Figure Lengend Snippet: Fig. 7. pA104R-specific IgG reactivity (A) pA104R-specific IgG antibody detection. (B) PEP15-specific IgG antibody detection. (C) PEP15-specific IgG1 and IgG2 antibody isotype detection. (D) IgG1/IgG2 ratio calculated for each IgG sample as the OD value of IgG1 divided by the OD value of IgG2. Red solid lines represent medians. DPI = days post-infection.

    Article Snippet: The plates were incubated with 1:200 diluted pig sera in 5% BSA in PBS for 1 h, followed by a blocking step with 5% BSA diluted in PBS for 1 h. The plates were then incubated with the 1:100 diluted polyclonal mouse anti-pig IgG1 antibody clone K139 3C8 (BioRad) or polyclonal mouse anti-pig IgG2 antibody clone K68 Ig2 (1:1000) (Bio-Rad) primary antibodies for 1 h, followed by incubation with the HRP-conjugated goat anti-mouse secondary antibody (1:5000) (BioRad) for an additional hour.

    Techniques: Infection

    Fig. 6. PEP-specific IgM and IgG antibody determinants. (A) PEP-specific IgM antibody detection in sera (n = 45) at a dilution of 1:250 was determined by peptide- based ELISA. Sera were categorized into non-infected or days post-infection (DPI) = 0 (n = 10), DPI = 7 (n = 10), DPI = 10 (n = 9), and DPI = 13–16 (n = 16). Red solid lines represent medians. (B) PEP-specific IgG antibody detection in sera (n = 48) at a dilution of 1:500 was determined by peptide-based ELISA. Sera were categorized into non-infected or DPI = 0 (n = 10), DPI = 13–16 (n = 16), DPI = 17–20 (n = 11), and DPI = 21–30 (n = 11). Red solid lines represent medians. (C) The pA104R dimeric structure (PDB 6LMH), generated using UCSF Chimera software. The IgM and IgG antibody determinants are indicated in brown (PEP23), and blue (PEP15).

    Journal: Antiviral research

    Article Title: Targeted mutagenesis of the β-strand DNA binding region of African swine fever virus histone-like protein (pA104R) impairs DNA-binding activity and antibody recognition.

    doi: 10.1016/j.antiviral.2023.105784

    Figure Lengend Snippet: Fig. 6. PEP-specific IgM and IgG antibody determinants. (A) PEP-specific IgM antibody detection in sera (n = 45) at a dilution of 1:250 was determined by peptide- based ELISA. Sera were categorized into non-infected or days post-infection (DPI) = 0 (n = 10), DPI = 7 (n = 10), DPI = 10 (n = 9), and DPI = 13–16 (n = 16). Red solid lines represent medians. (B) PEP-specific IgG antibody detection in sera (n = 48) at a dilution of 1:500 was determined by peptide-based ELISA. Sera were categorized into non-infected or DPI = 0 (n = 10), DPI = 13–16 (n = 16), DPI = 17–20 (n = 11), and DPI = 21–30 (n = 11). Red solid lines represent medians. (C) The pA104R dimeric structure (PDB 6LMH), generated using UCSF Chimera software. The IgM and IgG antibody determinants are indicated in brown (PEP23), and blue (PEP15).

    Article Snippet: The plates were incubated with 1:200 diluted pig sera in 5% BSA in PBS for 1 h, followed by a blocking step with 5% BSA diluted in PBS for 1 h. The plates were then incubated with the 1:100 diluted polyclonal mouse anti-pig IgG1 antibody clone K139 3C8 (BioRad) or polyclonal mouse anti-pig IgG2 antibody clone K68 Ig2 (1:1000) (Bio-Rad) primary antibodies for 1 h, followed by incubation with the HRP-conjugated goat anti-mouse secondary antibody (1:5000) (BioRad) for an additional hour.

    Techniques: Peptide ELISA, Infection, Generated, Software